Derivation of porcine circovirus type 2-negative pigs from positive breeding herds
نویسندگان
چکیده
Methods: Sow herds were screened for antibodies to PCV2 by ELISA, with sample-to-positive (S:P) ratios ≥ 0.2 considered positive. Piglets were obtained by segregated early weaning (SEW) at 10 to 12 days of age. Maternal anti-PCV2 antibody was determined by testing weekly blood samples by ELISA and indirect immunofluorescence assay. Absence of PCV2 was determined by polymerase chain reaction (PCR) on weekly serum samples and by PCR and immunohistochemistry assays on lymphoid tissues collected at necropsy (8 to 15 weeks of age).
منابع مشابه
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تاریخ انتشار 2003